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Effect of Sulfur and Bradyrhizobium Inoculation on Nodulation and Yield of Soybean (Glycine max L.) on Nitisols of Southwestern Ethiopia

Received: 19 October 2021    Accepted: 17 November 2021    Published: 29 December 2021
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Abstract

A field experiment was conducted at Jimma Agricultural Research Center (JARC) during 2017/18 growing season to evaluate the effect of integrated use of Bradyrhizobium strain and sulfur fertilization on nodulation and yield of soybean. The experiment consisted of four levels of S (0, 20, 30 and 40 kg ha-1) and three Bradyrhizobium strains (MAR-1495, SB-12 and Murdock) arranged factorially in completely randomized block design (RCBD) with three replications. Grain and straw yield increased due to inoculation of Bradyrhizobium strain whether used alone or in combination with S. Nodulation, parameters (nodule number per plant and nodule dry weight) and number of pods per plant were highly significantly influenced due to combined use of Bradyrhizobium strains with sulfur fertilization. The yield and yield components namely (grain yield, hundred seed weight, biomass yield and harvest index) were highly significantly (P < 0.01) affected by individual application of sulfur and inoculation of Bradyrhizobium strains alone. Accordingly, the highest grain yield (1496.35 kg ha-1) was obtained from application of S at a rate of 30 kg ha-1 corresponding 30.96% yield advantages compared with control and 1548.55 kg ha-1 grain yield was recorded due to inoculation with Murdock strain corresponding to 30.32% increase over MAR-1495 strain. The results clearly suggested that proper application of S along with Bradyrhizobium strain affect nodulation, grain and straw yield of soybean.

Published in Agriculture, Forestry and Fisheries (Volume 10, Issue 6)
DOI 10.11648/j.aff.20211006.18
Page(s) 280-287
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Sulfur, Strain, Nodulation, Yield

References
[1] Abaidoo R. C., Keyser H. H., Singleton P. W., Dashiell, K. E. and Sanginga, N. (2007). Population size distribution and symbiotic characteristics of indigenous Bradyrhizobium spp. that nodulate TGx soybean genotypes in Africa. App. Soil Ecol. 35: 57-67.
[2] Agricultural Transformation Agency (ATA) (2013). Status of soil resources in Ethiopia and priorities for sustainable management. GSP for Eastern and Southern Africa. Mar 25-27, 2013, Nairobi, Kenya.
[3] Anandham R, Sridarb R, Nalayinic P, Poonguzhalia S, Madhaiyana M, Tongmin SAA (2007) Potential for plant growth promotion in groundnut (Arachis hypogaea L.) cv. ALR-2 by co-inoculation of sulfur-oxidizing bacteria and Rhizobium. Microbiol. Res. 162: 139-153.
[4] Barney Jr PE, Bush LP (1985) Interaction of nitrate and sulphate reduction in tobacco. I. influence of availability of nitrate and sulphate. J Plant Nutr 8: 507-515.
[5] Bray R. H. and L. T. Kurtz. 1945. Determination of total organic and available forms of phosphorous in soils. Journal of Soil Science, 63: 370 – 379.
[6] Bremner JM, Mulvaney CS. Total Nitrogen In: AL Page, RH Miller, DR Keeney (Eds.) Methods of soil analysis. Part 2, Agronomy. 1982; 9: 595-624.
[7] Cazzato E, Laudadio V, Stellacci AM, Ceci E, Tufarelli V (2012). Influence of S application on protein quality, fatty acid composition and nitrogen fixation of white lupin (Lupinus albus L.). Eur. Food. Res. Technol. 235: 963-969.
[8] SAS (Statistical Analysis System). (2012). SAS 9.3 Macro Language: Reference. Cary, NC: SAS Institute.
[9] Dhage SJ, Patil VD, Patange MJ (2014). Effect of various levels of phosphorus and sulfur on yield, plant nutrient content, uptake and availability of nutrients at harvest stages of soybean [Glycine max (L.)]. Int. J. Curr. Microbiol. Appl. Sci. 3 (12): 833-844.
[10] Giller K. E. (2001). Nitrogen fixation in tropical cropping systems. 2nd edn. Wallingford, United Kingdom, CAB International. 423 pp.
[11] Gurmu F., Mohammed, H. and Alemaw G., 2009. Genotype x environment interactions and stability of soybean for grain yield and nutrition quality. African Crop Science Journal, 17 (2).
[12] Habtemichial KH, Singh BR, Aune JB (2007). Wheat response to N2 fixed by Fababean (Vicia faba L.) as affected by sulfur fertilization and rhizobial inoculation in semi-arid Northern Ethiopia. J. Plant Nutr. Soil Sci. 170: 412-418.
[13] Hussain K, Islam M, Siddique MT, Hayat R, Mohsan S (2011). Soybean growth and nitrogen fixation as affected by sulfur fertilization and inoculation under rain fed conditions in Pakistan. Int. J. Agric. Biol. 13: 951-955.
[14] Jamal A, Fazli SI, Ahmad S, Abdin MZ, Yun SJ (2005). Effect of Sulfur and Nitrogen application on Growth Characteristics, Seed and Oil Yields of Soybean Cultivars. Korean J. Crop Sci. 50 (5): 340-345.
[15] Jamal A, Moon YS, Abdin MZ (2010). Enzyme activity assessment of peanut (Arachis hypogeal L.) under slow-release S fertilization. Aust. J. Crop Sci. 4 (3): 169-174.
[16] Kuykendall, F. M. Hashem, and W. J. Hunter, “Enhanced competitiveness of a Bradyrhizobium japonicum mutant strain improved for nodulation and nitrogen fixation,” Plant and Soil, vol. 186, no. 1, pp. 121–125, 1996.
[17] Lindermann and G. E. Ham, “Soybean plant growth, nodulation, and nitrogen fixation as affected by root temperature,” Soil Science Society of America Journal, vol. 43, no. 6, pp. 1134–1140, 1979.
[18] Murphy J, Rilly J. A modified single solution method for the determination of phosphate in natural waters. Anal chim. Acta. 1962; 27: 31-36.
[19] Nelson, D. W. and L. E. Sommers, 1982 Total carbon, organic carbon, and organic matter. Methods of soil analysis part 3-chemical methods, (methodsofsoilan3), pp. 961-1010.
[20] Okalebo JR, Gathua KW, Woormer PL. Laboratory methods of soil and plant analysis: A working manual. 2nd Edition. TSBF-CIAT 2002; 128.
[21] Sanginga, N., Okogun, J. A., Vanlauwe, B. and Dashiell, K (2002). The contribution of nitrogen by promiscuous soybeans to maize-based cropping in the moist savanna of Nigeria. Plant and Soil 241: 223-231.
[22] Scherer HW, Pacyna S, Spoth K, Schulz M (2006). Sulfur supply to peas (Pisum sativum L.) influences symbiotic N2 fixation. Plant Soil Environ. 52 (2): 72-77.
[23] Singleton, H. M. Abdelmagid, and J. W. Tavares, “Effect of phosphorus on the effectiveness of strains of Rhizobium japonicum,” Soil Science Society of America Journal, vol. 49, no. 3, pp. 613–616, 1985.
[24] Solomon T, Pant LM, Angaw T (2012). Effects of Inoculation by Bradyrhizobium japonicum Strains on Nodulation, Nitrogen Fixation, and Yield of Soybean (Glycine max L.) Varieties on Nitisols of Bako, Western Ethiopia. ISRN Agronomy (261475): 1-8.
[25] Solomon, T., Lalit, M. P., and Tsige, A. (2012). Effects of inoculation by Bradyrhizobium strains on nodulation, nitrogen fixation and yield of soybean (Glycine max L. Merill) varieties on Nitisols of Bako, Western Ethiopia. Department of natural resource management, college of agric and env’tal science, Haramaya University. International scholarly research network.
[26] Somasegaran P, Hoben HJ (1994). Handbook for Rhizobia–Methods in Legume-Rhizobium Technology. Springer-Verlag, Heidelberg, Germany. pp. 10-52.
[27] Subba Rao, K. V. B. R. Tilak and C. S. Singh, “Dual inoculation with Rhizobium sp. and Glomus fasciculatum enhances nodulation, yield and nitrogen fixation in chickpea (Cicer arietinum Linn.),” Plant and Soil, vol. 95, no. 3, pp. 351–359, 1986.
[28] Tan Q, Zhang L, Grant J, Cooper P, Tegeder M (2010). Increased Phloem Transport of S-Methyl methionnine Positively Affects Sulfur and Nitrogen Metabolism and Seed Development in Pea Plants. Plant Physiol. 15: 1886-1896.
[29] Vanlauwe B, Descheemaeker K, Giller KE, Huising J, Merckx R, Nziguheba G, Wendt J, Zingore S (2015). Integrated soil fertility management in sub-Saharan Africa: unraveling local adaptation. Soil 1: 491-508.
[30] Wijnands, J. H. M., Gurmesa, N. D., Lute, J. C. M. and Van Loo, E. N., 2011. Ethiopian soya bean and sunflower value chains: Opportunities and challenges (No. 2011-016). LEI, part of Wageningen UR.
[31] Wu and Y. Arima, “Effect of Rhizobium inoculation and application of N, P, K fertilizer on the growth and nitrogen fixation of field-grown Chinese milk vetch,” Soil Science and Plant Nutrition, vol. 38, no. 1, pp. 75–84, 1992.
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    Habetamu Getinet, Gebreslassie Hailu, Hirut Birhanu. (2021). Effect of Sulfur and Bradyrhizobium Inoculation on Nodulation and Yield of Soybean (Glycine max L.) on Nitisols of Southwestern Ethiopia. Agriculture, Forestry and Fisheries, 10(6), 280-287. https://doi.org/10.11648/j.aff.20211006.18

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    ACS Style

    Habetamu Getinet; Gebreslassie Hailu; Hirut Birhanu. Effect of Sulfur and Bradyrhizobium Inoculation on Nodulation and Yield of Soybean (Glycine max L.) on Nitisols of Southwestern Ethiopia. Agric. For. Fish. 2021, 10(6), 280-287. doi: 10.11648/j.aff.20211006.18

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    AMA Style

    Habetamu Getinet, Gebreslassie Hailu, Hirut Birhanu. Effect of Sulfur and Bradyrhizobium Inoculation on Nodulation and Yield of Soybean (Glycine max L.) on Nitisols of Southwestern Ethiopia. Agric For Fish. 2021;10(6):280-287. doi: 10.11648/j.aff.20211006.18

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  • @article{10.11648/j.aff.20211006.18,
      author = {Habetamu Getinet and Gebreslassie Hailu and Hirut Birhanu},
      title = {Effect of Sulfur and Bradyrhizobium Inoculation on Nodulation and Yield of Soybean (Glycine max L.) on Nitisols of Southwestern Ethiopia},
      journal = {Agriculture, Forestry and Fisheries},
      volume = {10},
      number = {6},
      pages = {280-287},
      doi = {10.11648/j.aff.20211006.18},
      url = {https://doi.org/10.11648/j.aff.20211006.18},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20211006.18},
      abstract = {A field experiment was conducted at Jimma Agricultural Research Center (JARC) during 2017/18 growing season to evaluate the effect of integrated use of Bradyrhizobium strain and sulfur fertilization on nodulation and yield of soybean. The experiment consisted of four levels of S (0, 20, 30 and 40 kg ha-1) and three Bradyrhizobium strains (MAR-1495, SB-12 and Murdock) arranged factorially in completely randomized block design (RCBD) with three replications. Grain and straw yield increased due to inoculation of Bradyrhizobium strain whether used alone or in combination with S. Nodulation, parameters (nodule number per plant and nodule dry weight) and number of pods per plant were highly significantly influenced due to combined use of Bradyrhizobium strains with sulfur fertilization. The yield and yield components namely (grain yield, hundred seed weight, biomass yield and harvest index) were highly significantly (P -1) was obtained from application of S at a rate of 30 kg ha-1 corresponding 30.96% yield advantages compared with control and 1548.55 kg ha-1 grain yield was recorded due to inoculation with Murdock strain corresponding to 30.32% increase over MAR-1495 strain. The results clearly suggested that proper application of S along with Bradyrhizobium strain affect nodulation, grain and straw yield of soybean.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Effect of Sulfur and Bradyrhizobium Inoculation on Nodulation and Yield of Soybean (Glycine max L.) on Nitisols of Southwestern Ethiopia
    AU  - Habetamu Getinet
    AU  - Gebreslassie Hailu
    AU  - Hirut Birhanu
    Y1  - 2021/12/29
    PY  - 2021
    N1  - https://doi.org/10.11648/j.aff.20211006.18
    DO  - 10.11648/j.aff.20211006.18
    T2  - Agriculture, Forestry and Fisheries
    JF  - Agriculture, Forestry and Fisheries
    JO  - Agriculture, Forestry and Fisheries
    SP  - 280
    EP  - 287
    PB  - Science Publishing Group
    SN  - 2328-5648
    UR  - https://doi.org/10.11648/j.aff.20211006.18
    AB  - A field experiment was conducted at Jimma Agricultural Research Center (JARC) during 2017/18 growing season to evaluate the effect of integrated use of Bradyrhizobium strain and sulfur fertilization on nodulation and yield of soybean. The experiment consisted of four levels of S (0, 20, 30 and 40 kg ha-1) and three Bradyrhizobium strains (MAR-1495, SB-12 and Murdock) arranged factorially in completely randomized block design (RCBD) with three replications. Grain and straw yield increased due to inoculation of Bradyrhizobium strain whether used alone or in combination with S. Nodulation, parameters (nodule number per plant and nodule dry weight) and number of pods per plant were highly significantly influenced due to combined use of Bradyrhizobium strains with sulfur fertilization. The yield and yield components namely (grain yield, hundred seed weight, biomass yield and harvest index) were highly significantly (P -1) was obtained from application of S at a rate of 30 kg ha-1 corresponding 30.96% yield advantages compared with control and 1548.55 kg ha-1 grain yield was recorded due to inoculation with Murdock strain corresponding to 30.32% increase over MAR-1495 strain. The results clearly suggested that proper application of S along with Bradyrhizobium strain affect nodulation, grain and straw yield of soybean.
    VL  - 10
    IS  - 6
    ER  - 

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Author Information
  • Ethiopian Institute of Agricultural Research, Debre Markos Agricultural Research Center, Debre Markos, Ethiopia

  • Ethiopian Institute of Agricultural Research, Jimma Agricultural Research Center, Jimma, Ethiopia

  • Ethiopian Institute of Agricultural Research, Jimma Agricultural Research Center, Jimma, Ethiopia

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